Abstract
Recent studies have shown that epidermal stem cells derived from the epidermis of are able to form hair follicles in the presence of hair follicle-inducing cells. Here we describe the method that we have used to isolate and cultivate epidermal stem cells.
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References
Blanpain C, Fuchs E (2019) Epidermal homeostasis: a balancing act of stem cells in the skin. Nat Rev Mol Cell Biol 10(3):207–217
Boyce ST et al (2002) Cultured skin substitutes reduce donor skin harvesting for closure of excised, full-thickness burns. Ann Surg 235(2):269–279
Ito M et al (2007) Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature 447(7142):316–320
Wang X et al (2016) Hair follicle and sebaceous gland de novo regeneration with cultured epidermal stem cells and skin-derived precursors. Stem Cells Transl Med 5(12):1695–1706
Wang X et al (2017) Macrophages induce AKT/beta-catenin-dependent Lgr5(+) stem cell activation and hair follicle regeneration through TNF. Nat Commun 8:14091
Bickenbach JR (2005) Isolation, characterization, and culture of epithelial stem cells. Methods Mol Biol 289:97–102
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Wang, X., Dong, S., Wu, Y. (2018). Isolation and Cultivation of Epidermal (Stem) Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_132
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DOI: https://doi.org/10.1007/7651_2018_132
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8869-3
Online ISBN: 978-1-4939-8870-9
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