Abstract
Cell-based therapies have become a popular approach in the field of regenerative medicine. Human fibroblast cells, one of the cell types widely used in clinical applications, have been used for skin regeneration and wound healing procedures. Furthermore, they are utilized for aesthetic purposes since fibroblasts lose their abilities such as collagen synthesis with age. Here, we describe detailed procedures for isolation, culture, cryopreservation, and preparation of fibroblasts derived from adult human skin as a final product under good manufacturing practice–compliant conditions.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mao AS, Mooney DJ (2015) Regenerative medicine: current therapies and future directions. Proc Natl Acad Sci 112:14452–14459. https://doi.org/10.1073/pnas.1508520112
Mansbridge JN, Liu K, Pinney RE, Patch R, Ratcliffe A, Naughton GK (1999) Growth factors secreted by fibroblasts: role in healing diabetic foot ulcers. Diabetes Obes Metab 1:265–279. https://doi.org/10.1046/j.1463-1326.1999.00032.x
Costa-Almeida R, Gomez-Lazaro M, Ramalho C, Granja PL, Soares R, Guerreiro SG (2014) Fibroblast-endothelial partners for vascularization strategies in tissue engineering. Tissue Eng Part A 21:1055–1065. https://doi.org/10.1089/ten.TEA.2014.0443
Nolte SV, Xu W, Rennekampff H-O, Rodemann HP (2008) Diversity of fibroblasts—a review on implications for skin tissue engineering. Cells Tissues Organs 187:165–176. https://doi.org/10.1159/000111805
Sekiya EJ, Forte A, de Bellis Kühn TIB, Janz F, Bydlowski SP, Alves A (2012) Establishing a stem cell culture laboratory for clinical trials. Rev Bras Hematol Hemoter 34(3):236–241. https://doi.org/10.5581/1516-8484.20120057
Whyte W (2010) Cleanroom technology: fundamentals of design, testing and operation. Wiley, Hoboken
De Sousa PA et al (2016) Development and production of good manufacturing practice grade human embryonic stem cell lines as source material for clinical application. Stem Cell Res 17:379–390. https://doi.org/10.1016/j.scr.2016.08.011
Lupatov AY, Vdovin AS, Vakhrushev IV, Poltavtseva RA, Yarygin KN (2015) Comparative analysis of the expression of surface markers on fibroblasts and fibroblast-like cells isolated from different human tissues. Bull Exp Biol Med 158:537–543. https://doi.org/10.1007/s10517-015-2803-2
Bieback K, Schallmoser K, Klüter H, Strunk D (2008) Clinical protocols for the isolation and expansion of mesenchymal stromal cells. Transfus Med Hemother 35:286–294. https://doi.org/10.1159/000141567
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media New York
About this protocol
Cite this protocol
Aydoğdu, N., Öztel, O.N., Karaöz, E. (2020). Isolation, Culture, Cryopreservation, and Preparation of Skin-Derived Fibroblasts as a Final Cellular Product Under Good Manufacturing Practice–Compliant Conditions. In: Turksen, K. (eds) Stem Cells and Good Manufacturing Practices. Methods in Molecular Biology, vol 2286. Humana, New York, NY. https://doi.org/10.1007/7651_2020_333
Download citation
DOI: https://doi.org/10.1007/7651_2020_333
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1326-9
Online ISBN: 978-1-0716-1327-6
eBook Packages: Springer Protocols