Abstract
Intracranial xenograft meningioma models are considered best because of their biological relevance in tumor location. Traditionally, difficulty in obtaining serial measurements and high costs hindered intracranial tumor research; however, with the development of bioluminescence imaging systems, serial measurements of intracranial xenografted immortal meningioma cell lines can now be completed in a noninvasive, cost-effective, and efficient manner. This method involves transfecting immortal meningioma cells with the firefly luciferase gene, which then expresses the luciferase enzyme. This enzyme oxidizes luciferin in a reaction that releases photon energy that can be easily measured and recorded, even after the transfected tumor cells have been implanted intracranially in a mouse model. The transfected cells replicate key macroscopic, microscopic, immunohistochemical, and ultrastructure features of aggressive meningiomas.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Louis DN, Scheithauer BW, Budka H, von Deimling A, Kepes JJ (2000) Meningiomas. In: Kleihues P, Cavenee WK (eds) World Health Organization Classification of Tumors: pathology and genetics of tumors of the nervous system. IARC, Lyon, France, pp 176–184
Al-Rodhan RF, Laws ER (1991) The history of intracranial meningiomas. In: Al-Mefty O (ed) Meningiomas. Raven, New York, pp 1–7
Gonzales MF (1995) Classification and pathogenesis of brain tumors. In: Laws ER, Kaye AH (eds) Brain tumors: an encyclopedic approach. Churchill Livingstone, Edinburgh, pp 31–45
Burger PC, Scheithauer BW, Vogel FS (2002) Surgical pathology of the nervous system and its coverings, 4th edn. Churchill Livingstone, New York
Simpson D (1957) The recurrence of intracranial meningiomas after surgical treatment. J Neurol Neurosurg Psychiatry 20:22–39
Greene HS, Arnold H (1945) The homologous and heterologous transplantation of brain and brain tumors. J Neurosurg 2:315–329
Medhkour A, Van Roey M, Sobel RA, Fingert HJ, Lee J, Martuza RL (1989) Implantation of human meningiomas into the subrenal capsule of the nude mouse. A model for studies of tumor growth. J Neurosurg 71:545–550
Rana MW, Pinkerton H, Thornton H, Nagy D (1977) Heterotransplantation of human glioblastoma multiforme and meningioma to nude mice. Proc Soc Exp Biol Med 155:85–88
Ueyama Y, Morita K, Ochiai C, Ohsawa N, Hata J, Tamaoki N (1978) Xenotransplantation of a human meningioma and its lung metastasis in nude mice. Br J Cancer 37:644–647
van Furth WR, Laughlin S, Taylor MD, Salhia B, Mainprize T, Henkelman M, Cusimano MD, Ackerley C, Rutka JT (2003) Imaging of murine brain tumors using a 1.5 Tesla clinical MRI system. Can J Neurol Sci 30:326–332
Ragel BT, Elam IL, Gillespie DL, Flynn JR, Kelly DA, Mabey D, Feng H, Couldwell WT, Jensen RL (2008) A novel model of intracranial meningioma in mice using luciferase-expressing meningioma cells. Laboratory investigation. J Neurosurg 108:304–310
Ragel BT, Couldwell WT, Gillespie DL, Wendland MM, Whang K, Jensen RL (2008) A comparison of the cell lines used in meningioma research. Surg Neurol 70:295–307, discussion 307
Rubin JB, Kung AL, Klein RS, Chan JA, Sun Y, Schmidt K, Kieran MW, Luster AD, Segal RA (2003) A small-molecule antagonist of CXCR4 inhibits intracranial growth of primary brain tumors. Proc Natl Acad Sci U S A 100: 13513–13518
Jung HW, Yoo H, Paek SH, Choi KS (2000) Long-term outcome and growth rate of subtotally resected petroclival meningiomas: experience with 38 cases. Neurosurgery 46:567–574, discussion, pp 574–575
Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS (2006) Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect. Neurosurgery 58:365–372
Acknowledgment
We thank Kristin Kraus, M.Sc., for her excellent editorial assistance.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Rainey, J., Jensen, R.L. (2012). Meningioma Models. In: MartÃnez Murillo, R., MartÃnez, A. (eds) Animal Models of Brain Tumors. Neuromethods, vol 77. Humana Press, Totowa, NJ. https://doi.org/10.1007/7657_2011_27
Download citation
DOI: https://doi.org/10.1007/7657_2011_27
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-208-7
Online ISBN: 978-1-62703-209-4
eBook Packages: Springer Protocols