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Virus Elimination by Meristem-Tip Culture

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Characterization of Plant Viruses

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Meristem-tip culture is one of the most widely used methods for virus elimination from infected plants and production of virus-free plants. Apical meristem culture is a proven means of clonal propagation and also for eliminating viruses from infected plants. It exhibits inherent genetic stability and tremendous growth potential, making it useful material for long-term storage of virus-free germplasm. Also, due to the small length of the meristem explant, this technique will have the potential for eliminating other pathogenic organisms in addition to viruses. Meristem culture is more effective in eliminating viruses when it is combined with thermotherapy and chemotherapy. Pre-treatment of infected plants with higher temperature and usage of antiviral agents in the meristem regeneration medium have been reported to be an effective method for the elimination of many plant viruses.

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Authors and Affiliations

  1. Division of Crop Protection, ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India

    Alangar Ishwara Bhat

  2. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Govind Pratap Rao

Authors
  1. Alangar Ishwara Bhat
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  2. Govind Pratap Rao
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Bhat, A.I., Rao, G.P. (2020). Virus Elimination by Meristem-Tip Culture. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_47

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  • DOI: https://doi.org/10.1007/978-1-0716-0334-5_47

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0333-8

  • Online ISBN: 978-1-0716-0334-5

  • eBook Packages: Springer Protocols

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