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Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors

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The Epithelial-to Mesenchymal Transition

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2179))

Abstract

In the early stages of Drosophila melanogaster (Drosophila) metamorphosis, a partial epithelial-mesenchymal transition (pEMT) takes place in the peripodial epithelium of wing imaginal discs. Blocking this pEMT results in adults with internalized wings and missing thoracic tissue. Using peripodial GAL4 drivers, GAL80ts temporal control, and UAS RNAi transgenes, one can use these phenotypes to screen for genes involved in the pEMT. Dominant modifier tests can then be employed to identify genetic enhancers and suppressors. To analyze a gene’s role in the pEMT, one can then visualize peripodial cells in vivo at the time of eversion within the pupal case using live markers, and by dissecting, fixing, and immunostaining the prepupae. Alternatively, one can analyze the pEMT ex vivo by dissecting out wing discs and culturing them in the presence of ecdysone to induce eversion. This can provide a clearer view of the cellular processes involved and permit drug treatments to be easily applied.

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Acknowledgments

We thank members of the Murray lab for advice and support, Vishal Chaturvedi for critical reading of the manuscript, and Isabelle Lohrey for experimental tests. This work was supported by a National Collaborative Grant from the National Breast Cancer Foundation, and a University of Melbourne RGSS grant to M.J.M.

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Author notes

  1. Sofia Golenkina and Rosemary Manhire-Heath contributed equally with all other contributors.

Authors and Affiliations

  1. School of BioSciences, University of Melbourne, Parkville, VIC, Australia

    Sofia Golenkina, Rosemary Manhire-Heath & Michael J. Murray

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  1. Sofia Golenkina
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  2. Rosemary Manhire-Heath
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  3. Michael J. Murray
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Correspondence to Michael J. Murray .

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Editors and Affiliations

  1. Department of Biomedical Science and Bateson Centre, University of Sheffield, Sheffield, UK

    Kyra Campbell

  2. Centre de Biologie du Développement (CBD) Centre de Biologie Intégrative (CBI), Université de Toulouse, Toulouse, France

    Eric Theveneau

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Golenkina, S., Manhire-Heath, R., Murray, M.J. (2021). Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors. In: Campbell, K., Theveneau, E. (eds) The Epithelial-to Mesenchymal Transition. Methods in Molecular Biology, vol 2179. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0779-4_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0779-4_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0778-7

  • Online ISBN: 978-1-0716-0779-4

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