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Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing

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DNA Modifications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2198))

Abstract

Methylated DNA immunoprecipitation is a large scale purification technique. It enables the isolation of methylated DNA fragments for subsequent locus-specific or genome-wide analysis. Here we describe an immunoprecipitation protocol using a monoclonal mouse anti 5-methyl-cytidine antibody followed by next-generation sequencing (MeDIP-Seq).

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Author information

Authors and Affiliations

  1. Center for Reproductive Biology, School of Biological Sciences, Washington State University, Pullman, WA, USA

    Millissia Ben Maamar, Ingrid Sadler-Riggleman, Daniel Beck & Michael K. Skinner

Authors
  1. Millissia Ben Maamar
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  2. Ingrid Sadler-Riggleman
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  3. Daniel Beck
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  4. Michael K. Skinner
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Corresponding author

Correspondence to Michael K. Skinner .

Editor information

Editors and Affiliations

  1. Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham, UK

    Alexey Ruzov

  2. School of Life Sciences, Queen’s Medical Centre, University of Nottingham, Nottingham, UK

    Martin Gering

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© 2021 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Ben Maamar, M., Sadler-Riggleman, I., Beck, D., Skinner, M.K. (2021). Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)-Sequencing. In: Ruzov, A., Gering, M. (eds) DNA Modifications. Methods in Molecular Biology, vol 2198. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0876-0_23

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  • DOI: https://doi.org/10.1007/978-1-0716-0876-0_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0875-3

  • Online ISBN: 978-1-0716-0876-0

  • eBook Packages: Springer Protocols

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