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Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos

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Epigenetic Reprogramming During Mouse Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2214))

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Abstract

The mouse preimplantation embryo is an excellent system for studying how mammalian cells organize dynamically into increasingly complex structures. Accessible to experimental and genetic manipulations, its normal or perturbed development can be scrutinized ex vivo by real-time imaging from fertilization to late blastocyst stage. High-resolution imaging of multiple embryos at the same time can be compromised by embryos displacement during imaging. We have developed an inexpensive and easy-to-produce imaging device that facilitates greatly the imaging of preimplantation embryo. In this chapter, we describe the different steps of production and storage of the imaging device as well as its use for live imaging of mouse preimplantation embryos expressing fluorescent reporters from genetically modified alleles or after in vitro transcribed mRNA transfer by microinjection or electroporation.

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Acknowledgments

We thank the Institut Pasteur, the Centre National de la Recherche Scientifique and the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE and ANR-14 CE11-0017 PrEpiSpec) for their support. The activity of the Biomaterials and Microfluidics core facility is partially funded by the Carnot MS program. We are grateful to all lab members for their help and in particular Gwendoline Tallec and Sylvain Bessonnard for their involvement in the design and validation of the initial version of the mouse Eggbox. We thank Jérôme Artus for critical reading of the manuscript.

Author information

Author notes

  1. Sandrine Vandormael-Pournin and Emmanuel Frachon contributed equally to this work.

Authors and Affiliations

  1. Early Mammalian Development and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Paris, France

    Sandrine Vandormael-Pournin & Michel Cohen-Tannoudji

  2. Group of Biomaterials and Microfluidics Core Facility, Institut Pasteur, Paris, France

    Emmanuel Frachon & Samy Gobaa

Authors
  1. Sandrine Vandormael-Pournin
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  2. Emmanuel Frachon
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  3. Samy Gobaa
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  4. Michel Cohen-Tannoudji
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Corresponding author

Correspondence to Michel Cohen-Tannoudji .

Editor information

Editors and Affiliations

  1. Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Paris, France

    Katia Ancelin

  2. Institut Curie, CNRS UMR3215/ INSERM U934, Paris Sciences & Lettres Research University (PSL), Institut de Ge´ne´tique Mole´culaire de Montpellier, Univ Montpellier, CNRS Montpellier, France, Paris, France

    Maud Borensztein

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Vandormael-Pournin, S., Frachon, E., Gobaa, S., Cohen-Tannoudji, M. (2021). Microfabricated Device for High-Resolution Imaging of Preimplantation Embryos. In: Ancelin, K., Borensztein, M. (eds) Epigenetic Reprogramming During Mouse Embryogenesis. Methods in Molecular Biology, vol 2214. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0958-3_2

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  • DOI: https://doi.org/10.1007/978-1-0716-0958-3_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0957-6

  • Online ISBN: 978-1-0716-0958-3

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