Abstract
Western blotting continues to be a workhorse assay in laboratories throughout the world. The utility, low cost and accessibility of western blotting have allowed the technique to remain in practice, despite being developed over 40 years ago. Advances in antibody specificity, chemiluminescent formulations, properties of fluorescent molecules and imaging techniques provide gains in sensitivity, dynamic range, and ease of use. Here we discuss such aspects for the users’ consideration when planning and executing western blots, to take full advantage of contemporary practices.
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Acknowledgments
Bio-Rad, Bio-Dot, Immun-Blot, Mini-PROTEAN, Mini Trans-Blot, Mini-Protean, Protean, and Trans-Blot are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions.
TGX Stain-Free Precast Gels are covered by U.S. Patent Numbers 7,569,130 and 8,007,646. Clarity Max Western ECL Substrate is manufactured by Cyanagen Srl and is the subject of patent application numbers US7855287, EP1950207, US9040252, AU2011202658, CA2742025, US8129136, and EP1962095, together with other equivalent granted patents and patent applications in other countries like CN102313732.
All content contained within this publication is from Bio-Rad Bulletin 7370. All figures are copyright of Bio-Rad Laboratories, Inc. All trademarks used herein are the property of their respective owner.
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Oh, K. (2021). Technical Considerations for Contemporary Western Blot Techniques. In: Posch, A. (eds) Proteomic Profiling. Methods in Molecular Biology, vol 2261. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1186-9_29
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DOI: https://doi.org/10.1007/978-1-0716-1186-9_29
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