Abstract
Fluorescence-based tools are invaluable in studying cellular functions. Traditional small molecule or protein-based fluorescent sensors have been widely used for the cellular imaging, but the choice of targets is still limited. Recently, fluorogenic RNA-based sensors gained lots of attention. This novel sensor system can function as a general platform for various cellular targets. Here, we describe the steps to rationally design, optimize, and apply fluorogenic RNA-based sensors, using the intracellular imaging of tetracycline in living E. coli cells as an example.
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Acknowledgments
The authors gratefully acknowledge the UMass Amherst start-up grant, NIH R01AI136789, NSF CAREER, and Alfred P. Sloan Research Fellowship to M. You. The authors also thank other members in the You Lab for useful discussion and valuable comments.
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Yu, Q., Zheng, R., Narayanan, M., You, M. (2021). Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging. In: Ponchon, L. (eds) RNA Scaffolds. Methods in Molecular Biology, vol 2323. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1499-0_11
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DOI: https://doi.org/10.1007/978-1-0716-1499-0_11
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