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Isolation and Functional Characterization of Fusobacterium nucleatum Bacteriophage

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The Oral Microbiome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2327))

Abstract

Bacteriophages are viruses that specifically lyse bacteria. They have demonstrated potential in applications as antibacterial agents in medicine, agriculture, and environmental remediation. Due to the complex and dynamic nature of the oral microbiome, antibiotic treatment of chronic, polymicrobial oral diseases may lead to dysbiosis. In these diseases, bacteriophages may provide targeted activity against oral bacteria without such disruption to the broader microbial community. In this chapter, we describe the methods for screening samples that may contain bacteriophages against oral pathogenic bacteria, and using the example of FNU1, the bacteriophage we isolated against Fusobacterium nucleatum, describe the process of bacteriophage purification and characterization.

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Author information

Authors and Affiliations

  1. Department of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Science, La Trobe University, Bendigo, VIC, Australia

    Mwila Kabwe, Heng Ku & Joseph Tucci

  2. Quadram Institute Bioscience, Norfolk, UK

    Teagan Brown

  3. Melbourne Dental School, Faculty of Medicine, Dentistry & Health Science, The University of Melbourne, Melbourne, VIC, Australia

    Stuart Dashper

Authors
  1. Mwila Kabwe
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  2. Teagan Brown
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  3. Heng Ku
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  4. Stuart Dashper
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  5. Joseph Tucci
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Corresponding author

Correspondence to Joseph Tucci .

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Editors and Affiliations

  1. College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA

    Guy R. Adami

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Kabwe, M., Brown, T., Ku, H., Dashper, S., Tucci, J. (2021). Isolation and Functional Characterization of Fusobacterium nucleatum Bacteriophage. In: Adami, G.R. (eds) The Oral Microbiome. Methods in Molecular Biology, vol 2327. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1518-8_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1518-8_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1517-1

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