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Characterization of Circular RNAs

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Long Non-Coding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2372))

Abstract

Accumulated lines of evidence have revealed that a large number of circular RNAs are produced in transcriptomes from fruit fly to mouse and human. Unlike linear RNAs shaped with 5′ cap and 3′ tail, circular RNAs are characterized by covalently closed loop structures without open terminals, thus required specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs (ciRNAs) derived from intron lariats and circular RNAs (circRNAs) produced from back spliced exons in human.

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Acknowledgements

This work was supported by 31730111, 91940303, 31925011, and 31725009 from the National Natural Science Foundation of China, China to L.Y. and L.L.C.

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Authors and Affiliations

  1. Department of Medicine and Pathology, Cancer Research Institute, Beth Israel Deaconess Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Yang Zhang

  2. CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China

    Li Yang

  3. School of Life Science and Technology, ShanghaiTech University, Shanghai, China

    Li Yang & Ling-Ling Chen

  4. State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China

    Ling-Ling Chen

Authors
  1. Yang Zhang
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  2. Li Yang
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  3. Ling-Ling Chen
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Corresponding authors

Correspondence to Li Yang or Ling-Ling Chen .

Editor information

Editors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA, USA

    Lin Zhang

  2. Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA, USA

    Xiaowen Hu

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© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zhang, Y., Yang, L., Chen, LL. (2021). Characterization of Circular RNAs. In: Zhang, L., Hu, X. (eds) Long Non-Coding RNAs. Methods in Molecular Biology, vol 2372. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1697-0_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1697-0_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1696-3

  • Online ISBN: 978-1-0716-1697-0

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