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Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners

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Vascular Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2375))

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Abstract

Conventional ultrasound with frequency (2–15 MHz) has been a global diagnostic and therapeutic tool in clinical medicine, and high-frequency ultrasound (>30 MHz) has been a powerful investigative device for preclinical studies such as cardiovascular research. In this chapter, we describe the use of conventional ultrasound with a 2.5–10 MHz transducer as an investigative device for the measurement/detection of blood flow in rabbit model. The chapter will describe the procedures for the preparation of sonographer, imaging locations, and the details of the rabbits used as well as detailed imaging steps for the preoperative, immediately after operation, and postoperative follow-up ultrasound for vascular surgery, using a vascular graft implantation as an example. We also provide useful notes to avoid pitfalls for successful imaging. The overall goal of this chapter is to deliver the steps in using low-cost, non-invasive, and highly versatile clinical ultrasound imaging in preclinical small animal testing.

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Acknowledgments

This work is supported by the National Institutes of Health (NIH R01 HL130274-01A1) and the Natural Sciences and Engineering Council of Canada (CREATE-509950-2018 and CREATE-528202-2019). Staff at the Central Animal Facility of the University of Waterloo for animal care, Dr. Filip Konecny for surgery, Sabrina Mattiassi, Sarah Wen-Hui Chan, Laura Edmonds, and John Tse for the transportation.

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Authors and Affiliations

  1. Regenerative Nanomedicine Lab, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada

    Aung Moe Zaw, Yuan Yao, YeJin Jeong & Evelyn K. F. Yim

  2. Schlegel Research Institute for Aging, Department of Electrical & Computer Engineering, University of Waterloo, Waterloo, ON, Canada

    Richard Shangguan, Billy Y. S. Yiu, Adrian J. Y. Chee & Alfred C. H. Yu

Authors
  1. Aung Moe Zaw
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  2. Richard Shangguan
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  3. Yuan Yao
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  4. YeJin Jeong
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  5. Billy Y. S. Yiu
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  6. Adrian J. Y. Chee
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  7. Alfred C. H. Yu
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  8. Evelyn K. F. Yim
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Corresponding author

Correspondence to Evelyn K. F. Yim .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA

    Feng Zhao

  2. Department of Biomedical Engineering, Columbia University, New York, NY, USA

    Kam W. Leong

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zaw, A.M. et al. (2022). Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners. In: Zhao, F., Leong, K.W. (eds) Vascular Tissue Engineering. Methods in Molecular Biology, vol 2375. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1708-3_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1708-3_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1707-6

  • Online ISBN: 978-1-0716-1708-3

  • eBook Packages: Springer Protocols

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