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High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock

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Plant Circadian Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2398))

Abstract

The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the genetic mechanisms that generate oscillations of gene expression over the course of the day. However, its use for the study of spatial regulation is often limited by resolution. Here, we describe a modified luciferase imaging method for the study of the Arabidopsis circadian clock across the plant at sub-tissue-level resolution.

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Acknowledgments

M.G. and J.L. are funded by the Gatsby Charitable Foundation (grant number GAT3395/GLC). A.H. is funded by the BBSRC (grant number BB/P016774/1).

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Authors and Affiliations

  1. Sainsbury Laboratory, University of Cambridge, Cambridge, UK

    Mark Greenwood & James C. W. Locke

  2. Department of Biochemistry, University of Cambridge, Cambridge, UK

    Mark Greenwood

  3. Earlham Institute, Norwich, UK

    Anthony J. W. Hall

Authors
  1. Mark Greenwood
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  2. Anthony J. W. Hall
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  3. James C. W. Locke
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Corresponding author

Correspondence to James C. W. Locke .

Editor information

Editors and Affiliations

  1. Department of Biology, University of Bielefeld, Bielefeld, Nordrhein-Westfalen, Germany

    Dorothee Staiger

  2. Department of Biology, University of York, Heslington, UK

    Seth Davis

  3. Department of Biology, University of York, Heslington, UK

    Amanda Melaragno Davis

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Greenwood, M., Hall, A.J.W., Locke, J.C.W. (2022). High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock. In: Staiger, D., Davis, S., Davis, A.M. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 2398. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1912-4_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1912-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1911-7

  • Online ISBN: 978-1-0716-1912-4

  • eBook Packages: Springer Protocols

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