Abstract
Bioluminescence enables the monitoring of spatiotemporal dynamics and activity of bacterial populations in planta. We here describe a procedure to use AgroLux, a bioluminescent Agrobacterium tumefaciens, as a tool to study bacterial responses upon agroinfiltration. The first method details how to transform bioluminescent AgroLux to carry binary plasmids of interests. Then, a simple agroinfiltration assay for in planta imaging of bioluminescence signals is presented. AgroLux assays will increase our understanding of plant-Agrobacterium interactions and plant immunity and improve molecular farming.
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Acknowledgments
This work was financially supported by ERC project 616449 “GreenProteases” (RvdH); H2020 project 760331 “Newcotiana” (PVJ); the Quebec Government’s research funding body FRQNT (PVJ); and BBSRC iCASE (ID).
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Jutras, P.V., Dodds, I., van der Hoorn, R.A.L. (2022). A Bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in Planta. In: Schillberg, S., Spiegel, H. (eds) Recombinant Proteins in Plants. Methods in Molecular Biology, vol 2480. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2241-4_15
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DOI: https://doi.org/10.1007/978-1-0716-2241-4_15
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