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Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases

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Multiplex Biomarker Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2511))

Abstract

Severe cases of SARS-CoV-2 and other pathogenic virus infections are often associated with the uncontrolled release of proinflammatory cytokines, known as a “cytokine storm.” We present a protocol for multiplex analysis of three cytokines, tumor necrosis factor-alpha (TNF-a), interleukin 6 (IL-6), and IL-10, which are typically elevated in cytokine storm events and may be used as a predictive biomarker profile of disease severity or disease course.

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Author information

Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

  2. Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mitra Abbasifard

  3. Department of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

    Mitra Abbasifard

  4. Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Tannaz Jamialahmadi

  5. Sabinsa Corporation, East Windsor, NJ, USA

    Muhammed Majeed

  6. Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India

    Prashant Kesharwani

  7. Applied Biomeical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Amirhossein Sahebkar

  8. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Amirhossein Sahebkar

  9. School of Medicine, The University of Western Australia, Perth, Australia

    Amirhossein Sahebkar

  10. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Amirhossein Sahebkar

Authors
  1. Paul C. Guest
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  2. Mitra Abbasifard
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  3. Tannaz Jamialahmadi
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  4. Muhammed Majeed
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  5. Prashant Kesharwani
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  6. Amirhossein Sahebkar
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Corresponding author

Correspondence to Amirhossein Sahebkar .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, University of Campinas, Campinas, Sao Paulo, Brazil

    Paul C. Guest

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Guest, P.C., Abbasifard, M., Jamialahmadi, T., Majeed, M., Kesharwani, P., Sahebkar, A. (2022). Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 2511. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2395-4_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2395-4_18

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2394-7

  • Online ISBN: 978-1-0716-2395-4

  • eBook Packages: Springer Protocols

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