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Determining miRNA Expression Patterns in Xenopus

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MicroRNA Detection and Target Identification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2630))

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Abstract

Whole-mount in situ hybridization (WISH) is a technique that enables temporal and spatial visualization of RNA molecules in an embryo or whole tissue by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25 nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in Xenopus laevis or tropicalis by WISH using two different approaches: LNA-WISH to visualize mature miRNAs and pri-miRNA-WISH to visualize the immature form of miRNAs, the pri-miRNAs.

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Acknowledgements

We would like to thank the members of the Wheeler and Munsterberg labs, both past and present, who have contributed to the development of these protocols.

Funding

MA is funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 860635. AMG was supported by UKRI Biotechnology and Biological Sciences Research Council Norwich Research Park Biosciences Doctoral Training Partnership grants (BB/M011216/1).

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Author notes

  1. Marco Antonaci and Alice M. Godden have contributed equally to this chapter

Authors and Affiliations

  1. School of Biological Sciences, University of East Anglia, Norwich, UK

    Marco Antonaci, Alice M. Godden & Grant N. Wheeler

Authors
  1. Marco Antonaci
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  2. Alice M. Godden
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  3. Grant N. Wheeler
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Corresponding author

Correspondence to Grant N. Wheeler .

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Editors and Affiliations

  1. RNA Biology, University of East Anglia, Norwich, UK

    Tamas Dalmay

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Antonaci, M., Godden, A.M., Wheeler, G.N. (2023). Determining miRNA Expression Patterns in Xenopus. In: Dalmay, T. (eds) MicroRNA Detection and Target Identification. Methods in Molecular Biology, vol 2630. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2982-6_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2982-6_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2981-9

  • Online ISBN: 978-1-0716-2982-6

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