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Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles

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Cell-Secreted Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2668))

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Abstract

Exosomes are small extracellular vesicles that contain RNA, lipids, and proteins and can act as cellular messengers, carrying information to cells and tissues in the body. Thus, sensitive, label-free, and multiplexed analysis of exosomes may help in early diagnosis of important diseases. Here, we describe the process of pretreatment of cell-derived exosomes, preparation of surface-enhanced Raman scattering (SERS) substrates, and label-free SERS detection of exosomes using sodium borohydride aggregators. This method can enable the observation of SERS signals of exosomes that are clear and stable and have a good signal-to-noise ratio.

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Acknowledgments

HMU Marshal Initiative Funding (No.: HMUMIF-21012); National Natural Science Foundation for Youth (No.: 82202648); Jian-An Huang acknowledges the DigiHealth-project, a strategic profiling project at the University of Oulu that is supported by the Academy of Finland (project number 326291) and the University of Oulu.

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Authors and Affiliations

  1. College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China

    Yang Li & Yunpeng Wang

  2. Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, The Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, Heilongjiang, China

    Jinwei Tian

  3. Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland

    Jian-An Huang

Authors
  1. Yang Li
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  2. Yunpeng Wang
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  3. Jinwei Tian
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  4. Jian-An Huang
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Corresponding author

Correspondence to Jian-An Huang .

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Editors and Affiliations

  1. GeneCellNano, Oulu University, Oulu, Finland

    Seppo Vainio

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Li, Y., Wang, Y., Tian, J., Huang, JA. (2023). Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles. In: Vainio, S. (eds) Cell-Secreted Vesicles. Methods in Molecular Biology, vol 2668. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3203-1_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3203-1_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3202-4

  • Online ISBN: 978-1-0716-3203-1

  • eBook Packages: Springer Protocols

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