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Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders

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Bone Marrow and Stem Cell Transplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1109))

Abstract

Relapse represents the main cause of treatment failure after stem cell transplantation (SCT). Thus, monitoring of minimal residual disease (MRD) in allografted patients allows an early detection of recurrence and a subsequent intervention prior to clinically detectable relapse. MRD assessment by polymerase chain reaction-based methods is currently part of the routine clinical management of patients with chronic myeloid leukemia after allo-SCT. It is also recognized that it is a useful prognostic tool in several mature lymphoid and plasma cell disorders such as chronic lymphocytic leukemia, follicular lymphoma, mantle cell lymphoma, and multiple myeloma. In some of these entities, clinical trials employing MRD as a decision-making tool are currently ongoing and will define whether sensitive MRD detection allows for earlier therapeutic intervention to improve the outcome of SCT. We here discuss the methods of MRD evaluation in lymphoid and plasma cell disorders following transplantation with the ultimate aim of providing critical information for the setup of molecular approaches to detect MRD.

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Author information

Authors and Affiliations

  1. Division of Hematology, Fondazione IRCCS Istituto Nazionale Tumori Milano, University of Milano, Milan, Italy

    Paolo Corradini

  2. Division of Hematology, Bone Marrow Transplantation Unit, Fondazione IRCCS Istituto Nazionale Tumori Milano, University of Milano, Milan, Italy

    Cristiana Carniti

Authors
  1. Paolo Corradini
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  2. Cristiana Carniti
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Editors and Affiliations

  1. Department of Hematology, Ankara University, Ankara, Turkey

    Meral Beksaç

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Corradini, P., Carniti, C. (2014). Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders. In: Beksaç, M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Biology, vol 1109. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9437-9_12

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  • DOI: https://doi.org/10.1007/978-1-4614-9437-9_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9436-2

  • Online ISBN: 978-1-4614-9437-9

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