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Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues

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Axon Growth and Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1162))

Abstract

Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.

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Acknowledgments

This work was supported by grants from the US Army W81XWH-12-1-0319 (KKP), NEI (KKP), and Ziegler Foundation (KKP). We thank the Imaging and Viral Vector Core at the Miami Project to Cure Paralysis.

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Authors and Affiliations

  1. Department of Neurosurgery, Miami Project to Cure Paralysis, Miller School of Medicine, University of Miami, Park Lab, LPLC, RM 3-01, 1095 NW 14th Terrace, Miami, FL, 33136, USA

    Xueting Luo, Benjamin Yungher & Kevin K. Park

Authors
  1. Xueting Luo
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  2. Benjamin Yungher
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  3. Kevin K. Park
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Corresponding author

Correspondence to Kevin K. Park .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, USA

    Andrew J. Murray

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© 2014 Springer Science+Business Media New York

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Luo, X., Yungher, B., Park, K.K. (2014). Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues. In: Murray, A. (eds) Axon Growth and Regeneration. Methods in Molecular Biology, vol 1162. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0777-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-0777-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0776-2

  • Online ISBN: 978-1-4939-0777-9

  • eBook Packages: Springer Protocols

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