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Measurement of Eosinophil Kinetics in Healthy Volunteers

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Eosinophils

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1178))

Abstract

Radiolabelled leukocyte scans are widely used in nuclear medicine to locate sites of infection and inflammation. Radiolabelling of leukocyte subpopulations can also yield valuable information on cell trafficking and kinetics in vivo, but care must be taken to minimize inadvertent cell activation ex vivo. Here, we describe the use of autologous indium111-labelled eosinophils to measure eosinophil intravascular life-span and monitor their distribution and fate using gamma camera imaging in healthy non-atopic individuals.

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Acknowledgements

This work was supported by Asthma UK [08/11]; the Medical Research Council [grant number MR/J00345X/1]; the Wellcome Trust [grant number 098351/Z/12/Z]; Biotechnology and Biological Sciences Research Council [grant number BB/H531100/1]; Papworth Hospital R&D; and Cambridge National Institute for Health Research Biomedical Research Centre.

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Authors and Affiliations

  1. Department of Medicine, Addenbrooke’s and Papworth Hospitals, University of Cambridge School of Clinical Medicine, Cambridge, CB2 0QQ, UK

    Neda Farahi, Chrystalla Loutsios, Rosalind P. Simmonds, Linsey Porter, Alison M. Condliffe & Edwin R. Chilvers

  2. Nuclear Medicine, Cambridge University Hospitals National Health Service Foundation Trust, Addenbrooke’s Hospital, Cambridge, CB2 0QQ, UK

    Daniel Gillett & Sarah Heard

  3. Clinical Imaging Sciences Centre, Brighton and Sussex Medical School, BN2 5BE, Brighton, UK

    A. Michael Peters

Authors
  1. Neda Farahi
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  2. Chrystalla Loutsios
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  3. Rosalind P. Simmonds
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  4. Linsey Porter
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  5. Daniel Gillett
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  6. Sarah Heard
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  7. A. Michael Peters
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  8. Alison M. Condliffe
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  9. Edwin R. Chilvers
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Corresponding author

Correspondence to Neda Farahi .

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Editors and Affiliations

  1. Division of Applied Medicine, University of Aberdeen Institute of Medical Sciences, Aberdeen, United Kingdom

    Garry M. Walsh

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© 2014 Springer Science+Business Media, New York

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Farahi, N. et al. (2014). Measurement of Eosinophil Kinetics in Healthy Volunteers. In: Walsh, G. (eds) Eosinophils. Methods in Molecular Biology, vol 1178. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1016-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-1016-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1015-1

  • Online ISBN: 978-1-4939-1016-8

  • eBook Packages: Springer Protocols

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