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Investigating TLR Signaling Responses in Murine Dendritic Cells Upon Bacterial Infection

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Host-Bacteria Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1197))

Abstract

Innate immune recognition of microbial components is critical for the onset of an appropriate immune response against invading pathogens, in particular by dendritic cells. Toll-like receptors (TLRs) are key in the detection of a variety of microbial stimuli.

Here we focus on the methodology used to evaluate the role of TLRs in the process of dendritic cell response to bacterial intracellular infections, using bone marrow-derived dendritic cells (BMDCs) as a model system. This protocol describes how to access the level of activation of BMDCs using standard immunology and biochemistry approaches along with examination of infected cells by immunofluorescence microscopy.

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Author information

Authors and Affiliations

  1. Bases Moléculaires et Structurales des Systèmes Infectieux, CNRS UMR 5086, Institut de Biologie et Chimie des Protéines, Université Lyon 1, IBCP, 69367, Lyon, France

    Suzana Pinto Salcedo

  2. Centre d’Immunologie de Marseille-Luminy, Aix-Marseille Université UM 2, 13288, Marseille, France

    Lena Alexopoulou

  3. INSERM U 1104, 13288, Marseille, France

    Lena Alexopoulou

  4. CNRS UMR 7280, 13288, Marseille, France

    Lena Alexopoulou

Authors
  1. Suzana Pinto Salcedo
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  2. Lena Alexopoulou
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Corresponding authors

Correspondence to Suzana Pinto Salcedo or Lena Alexopoulou .

Editor information

Editors and Affiliations

  1. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

    Annette C. Vergunst

  2. U1047, UFR Médecine, INSERM, Université Montpellier 1, Nîmes, France

    David O'Callaghan

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© 2014 Springer Science+Business Media New York

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Salcedo, S.P., Alexopoulou, L. (2014). Investigating TLR Signaling Responses in Murine Dendritic Cells Upon Bacterial Infection. In: Vergunst, A., O'Callaghan, D. (eds) Host-Bacteria Interactions. Methods in Molecular Biology, vol 1197. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1261-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1261-2_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1260-5

  • Online ISBN: 978-1-4939-1261-2

  • eBook Packages: Springer Protocols

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