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High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach

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Protein Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1216))

Abstract

Protein combinatorial libraries have become a platform technology for exploring protein sequence space for novel molecules for use in research, synthetic biology, biotechnology, and medicine. To expedite the isolation of proteins with novel/desired functions using screens and selections, high-quality approaches that generate protein libraries rich in folded and soluble structures are desirable for this goal. The binary patterning approach is a protein library design method that incorporates elements of both rational design and combinatorial diversity to specify the arrangement of polar and nonpolar amino acid residues in the context of a desired, folded tertiary structure template. An overview of the considerations necessary to design and construct binary patterned libraries of de novo and natural proteins is presented.

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Acknowledgements

Support was obtained from the following sources: a pilot project grant from the NIH National Center for Research Resources (NCRR) Grant P20 RR020171, the Kentucky Science and Engineering Foundation (Grant Agreement # KSEF-148-502-207-201 with the Kentucky Science and Technology Corporation), the University of Kentucky Office of the Vice President of Research, and the University of Kentucky College of Medicine Startup funds.

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Authors and Affiliations

  1. Departments of Anatomy and Neurobiology, Molecular and Cellular Biochemistry, and the Center of Structural Biology, University of Kentucky College of Medicine, MN225 Chandler Medical Center, 800 Rose Street, Lexington, KY, 40536-0298, USA

    Luke H. Bradley

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  1. Luke H. Bradley
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Correspondence to Luke H. Bradley .

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Editors and Affiliations

  1. Department of Chemistry, University of Basel, Switzerland

    Valentin Köhler

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Bradley, L.H. (2014). High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach. In: Köhler, V. (eds) Protein Design. Methods in Molecular Biology, vol 1216. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1486-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-1486-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1485-2

  • Online ISBN: 978-1-4939-1486-9

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