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Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon Enteropathogenic Escherichia coli Infection

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Label-Free Biosensor Methods in Drug Discovery

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

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Abstract

Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

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Acknowledgments

VY deeply thanks Dan Davidov (HUJI) and Michael Golosovsky (HUJI) for their scientific guidance. We also thank Leorah Kharilker (HUJI), Efrat Zlotkin-Rivkin (HUJI), and Vladislav Lirtsman (HUJI) for helping with experiments; Ilan Rosenshine (HUJI) and Michael Donnenberg (UMD) for providing EPEC strains. BA acknowledges the support from the Start-Up grant Tack A from Yissum, the Israel Science Foundation (ISF), funded by the Israel Academy of Sciences (Grants 1167/08 and 1483/13), and a grant from the Israel Cancer Association.

Author information

Authors and Affiliations

  1. Institute of Biochemistry, Food Science and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 12, Rehovot, 7610001, Israel

    Victor Yashunsky

  2. Department of Cell and Developmental Biology, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel

    Benjamin Aroeti

Authors
  1. Victor Yashunsky
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  2. Benjamin Aroeti
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Corresponding author

Correspondence to Victor Yashunsky .

Editor information

Editors and Affiliations

  1. Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York, USA

    Ye Fang

Glossary

EPEC

Enteropathogenic Escherichia coli

IR

Infrared

L x

Surface plasmon propagation length

MDCK

Madin-Darby canine kidney

SP

Surface plasmon

SPR

Surface plasmon resonance

T3SS

Type-III secretion system

TJ

Tight junction

TM

Transverse magnetic

δ z

Surface plasmon penetration depth

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Yashunsky, V., Aroeti, B. (2015). Infrared Surface Plasmon Spectroscopy Decodes Early Processes in Epithelial Host Cells upon Enteropathogenic Escherichia coli Infection. In: Fang, Y. (eds) Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2617-6_20

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  • DOI: https://doi.org/10.1007/978-1-4939-2617-6_20

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2616-9

  • Online ISBN: 978-1-4939-2617-6

  • eBook Packages: Springer Protocols

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