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Cationic Electrophoresis and Eastern Blotting

  • Protocol
Western Blotting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1312))

Abstract

Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis, staining, and blotting of such gels are presented.

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Acknowledgement

This work was supported in part by Kuwait University grant MPB029.

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Authors and Affiliations

  1. Ross University School of Medicine, 266, Roseau, Dominica, West Indies

    Engelbert Buxbaum Dr. rer. nat., Dipl. Biol.

  2. ∎, Weezer Str. 197, Kevelaer, 47623, Germany

    Engelbert Buxbaum Dr. rer. nat., Dipl. Biol.

Authors
  1. Engelbert Buxbaum Dr. rer. nat., Dipl. Biol.
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Corresponding author

Correspondence to Engelbert Buxbaum Dr. rer. nat., Dipl. Biol. .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Buxbaum, E. (2015). Cationic Electrophoresis and Eastern Blotting. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_19

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  • DOI: https://doi.org/10.1007/978-1-4939-2694-7_19

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2693-0

  • Online ISBN: 978-1-4939-2694-7

  • eBook Packages: Springer Protocols

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