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Genetic Tools to Study T Cell Development

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T-Cell Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1323))

Abstract

Genetics tools, and especially the ability to enforce, by transgenesis, or disrupt, by homologous recombination, gene expression in a cell-specific manner, have revolutionized the study of immunology and propelled the laboratory mouse as the main model to study immune responses. Perhaps more than any other aspect of immunology, the study of T cell development has benefited from these technologies. This brief chapter summarizes genetic tools specific to T cell development studies, focusing on mouse strains with lineage- and stage-specific expression of the Cre recombinase, or expressing unique antigen receptor specificities. It ends with a broader discussion of strategies to enforce ectopic lineage and stage-specific gene expression.

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Authors and Affiliations

  1. Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Thomas Ciucci & Melanie S. Vacchio

  2. Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 3032A, Bethesda, MD, 20892, USA

    Rémy Bosselut

Authors
  1. Thomas Ciucci
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  2. Melanie S. Vacchio
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  3. Rémy Bosselut
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Correspondence to Rémy Bosselut .

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Editors and Affiliations

  1. Laboratory of Immune Cell Biology, National Institutes of Health National Cancer Institute, Bethesda, Maryland, USA

    Rémy Bosselut

  2. Laboratory of Immune Cell Biology, National Institutes of Health National Cancer Institute, Bethesda, Maryland, USA

    Melanie S. Vacchio

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Ciucci, T., Vacchio, M.S., Bosselut, R. (2016). Genetic Tools to Study T Cell Development. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2809-5_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2808-8

  • Online ISBN: 978-1-4939-2809-5

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