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Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amplification: An Overview

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Whole Genome Amplification

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1347))

Abstract

Single cells are increasingly used to determine the heterogeneity of therapy targets in the genome during the course of a disease. The first challenge using single cells is to isolate these cells from the surrounding cells, especially when the targeted cells are rare. A number of techniques have been developed for this goal, each having specific limitations and possibilities. In this chapter, five of these techniques are discussed in the light of the isolation of circulating tumor cells (CTC) present at extremely low frequency in the blood of patients with metastatic cancer from the perspective of pre-enriched samples by means of CellSearch. The techniques described are micromanipulation, FACS, laser capture microdissection, DEPArray, and microfluidic solutions. All platforms are hampered with a low efficiency and differences in hands-on time and costs are the most important drivers for selection of the optimal platform.

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Authors and Affiliations

  1. Department of Medical Cell BioPhysics, Faculty of Science and Technology, MIRA Research Institute, University of Twente, 217, Drienerlolaan 5, 7500 AE, Enschede, The Netherlands

    Joost F. Swennenhuis & Leon Terstappen

Authors
  1. Joost F. Swennenhuis
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  2. Leon Terstappen
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Corresponding author

Correspondence to Joost F. Swennenhuis .

Editor information

Editors and Affiliations

  1. Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria

    Thomas Kroneis

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Swennenhuis, J.F., Terstappen, L. (2015). Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amplification: An Overview. In: Kroneis, T. (eds) Whole Genome Amplification. Methods in Molecular Biology, vol 1347. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2990-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2990-0_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2989-4

  • Online ISBN: 978-1-4939-2990-0

  • eBook Packages: Springer Protocols

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