Abstract
A technique is described for synthesizing and transfecting double stranded RNA (dsRNA) for RNA interference (RNAi) in Sf-21 cell culture. Transfection with dsRNA only requires an hour and the cells usually recover within 12 h. Suggestions for designing dsRNA are included in the methods. Furthermore, websites are provided for rapid and effective dsRNA design. Three kits are essential for using the described methods: RNAqueous®-4PCR, Megascript™ T7 kit, and the Superscript™ III kit from Life Technologies, Inc.
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Wu, HC., March, J.C., Bentley, W.E. (2016). Gene Silencing in Insect Cells Using RNAi. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_24
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DOI: https://doi.org/10.1007/978-1-4939-3043-2_24
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3042-5
Online ISBN: 978-1-4939-3043-2
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