Abstract
Viral vector delivery of RNA silencing constructs, when administered into vasculature, typically results in poor central nervous system (CNS) transduction due to the inability of the vector to cross the blood–brain barrier (BBB). However, adeno-associated virus serotype 9 (AAV9) has the ability to cross the BBB and robustly transduce brain parenchyma and peripheral tissues at biologically meaningful levels when injected intravenously. Recent work by our lab has shown that this method can be used to deliver RNA silencing constructs, resulting in significant reductions in gene expression in multiple brain regions and in peripheral tissues. Here, we outline a method for delivery of AAV9 vectors expressing RNA interference (RNAi) constructs that lead to robust simultaneous transduction of mouse peripheral tissues and the CNS following a single injection into the jugular vein. Additionally, we outline methods for necropsy and immunofluorescence to detect AAV9 transduction patterns in the rodent CNS following a vascular delivery.
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Acknowledgments
This research was supported by a research grant from the Hereditary Disease Foundation (J.L.M.), ONPRC Core Grants RR000163 (J.L.M.) and P51OD011092 (J.L.M.), and a T32 Neuroscience training grant NS7466-14 (B.D.D.). Confocal microscopy was supported by grants S10RR024585 and P30-NS061800.
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Dufour, B.D., McBride, J.L. (2016). Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery. In: Shum, K., Rossi, J. (eds) SiRNA Delivery Methods. Methods in Molecular Biology, vol 1364. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3112-5_21
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DOI: https://doi.org/10.1007/978-1-4939-3112-5_21
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3111-8
Online ISBN: 978-1-4939-3112-5
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