Abstract
The analysis of a large number of two-dimensional gel electrophoresis (2-DE) images requires developing automatic methods. In such analyses, spot matching plays a fundamental role, in particular for the identification of proteins. We describe a simple and accurate method which allows to automatically and accurately match spots in 2-DE images. The method consists of simultaneously exploiting the distance between the spots, their intensity, and the pattern formed by their spatial configuration.
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Acknowledgement
The authors thank the team of Dr. Long-Fei WU, Laboratory of Bacterial Chemistry, CNRS URP9034, Marseille, France, for providing the 2-DE image s and the ground truth. Yuemin ZHU was partly supported by the French ANR under ANR-13-MONU-0009-01. Hua-Mei Xin was partly supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars (2009- 36).
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Xin, HM., Zhu, Y. (2016). Spot Matching of 2-DE Images Using Distance, Intensity, and Pattern Information. In: Marengo, E., Robotti, E. (eds) 2-D PAGE Map Analysis. Methods in Molecular Biology, vol 1384. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3255-9_7
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DOI: https://doi.org/10.1007/978-1-4939-3255-9_7
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