Abstract
Arabidopsis thaliana was the first higher organism to have its genome sequenced and is now widely regarded as the model dicot. Like all plants, Arabidopsis develops distinct growth patterns in response to different environmental stimuli. This can be seen in the gravitropic response of roots. Methods to investigate this particular tropism are presented here. First, we describe a high-throughput time-lapse photographic analysis of root growth and curvature response to gravistimulation allowing the quantification of gravitropic kinetics and growth rate at high temporal resolution. Second, we present a protocol that allows a quantitative evaluation of gravitropic sensitivity using a homemade 2D clinostat. Together, these approaches allow an initial comparative analysis of the key phenomena associated with root gravitropism between different genotypes and/or accessions.
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References
Galvan Ampudia C, Julkowska M, Darwish E, Gandullo J, Korver R et al (2013) Halotropism Is a response of plant roots to avoid a saline environment. Curr Biol 23:204450
Massa G, Gilroy S (2003) Touch modulates gravity sensing to regulate the growth of primary roots of Arabidopsis thaliana. Plant J 33:43545
Takahashi N, Yamazaki Y, Kobayashi A, Higashitani A, Takahashi H (2003) Hydrotropism interacts with gravitropism by degrading amyloplasts in seedling roots of Arabidopsis and radish. Plant Physiol 132(805):10
Baldwin K, Strohm A, Masson P (2013) Gravity sensing and signal transduction in vascular plant primary roots. Am J Bot 100(1):126–142
Strohm A, Baldwin K, Masson P (2012) Molecular mechanisms of root gravity sensing and signal transduction. Wiley Interdiscip Rev Dev Biol 1:276–285
Strohm A, Baldwin K, Masson P (2012) Multiple roles for membrane-associated protein trafficking and signaling in gravitropism. Front Plant Sci 3:274
Larsen P (1962) Orthogravitropism in roots. In: Ruhland W (ed) Encyclopedia of plant physiol, Vol 17, Part 2, physiology of movements. Springer-Verlag, Berlin, pp 153–199
Perbal G, Jeune B, Lefranc A, Carnero-Diaz E, Driss-Ecole D (2002) The dose-response curve of the gravitropic reaction: a re-analysis. Physiol Plant 114:336–342
Young L, Harrison B, Murthy N, Moffatt B, Gilroy S, Masson P (2006) Adenosine kinase modulates root gravitropism and cap morphogenesis in Arabidopsis thaliana. Plant Physiol 142:564–573
Brunoud G, Wells DM, Oliva M, Larrieu A, Mirabet V, Burrow AH, Beeckman T et al (2012) A novel sensor to map auxin response and distribution at high spatio-temporal resolution. Nature 482(7383):103–106
Brooks T, Miller N, Spalding E (2010) Plasticity of Arabidopsis root Gravitropism throughout a multidimensional condition space quantified by automated image analysis. Plant Physiol 152(1):206–216
Wells D, French A, Naeem A, Ishaq O, Traini R, Hijazi H, Bennett M, Pridmore T (2012) Recovering the dynamics of root growth and development using novel image acquisition and analysis methods. Philos Trans R Soc B: Biol Sci 367(1595):1517–1524
Sangari A, Ohme-Takagi M, Mitsuda N, Assadi A (2012) Automated imaging & high-throughput phenotyping, massively parallel image acquisition, analysis and modeling: mapping plant genotype-phenotype relation. Cold Spring Harbor, Cold Spring Harbor, NY
Russino A, Ascrizzi A, Popova L, Tonazzini A, Mancuso S, Mazzolai B (2013) A novel tracking tool for the analysis of plant-root tip movements. Bioinspir Biomim 8:025004
Buer CS, Wasteneys GO, Masle J (2003) Ethylene modulates root-wave responses in Arabidopsis. Plant Physiol 132:1085–1096
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Barker, R., Cox, B., Silber, L., Sangari, A., Assadi, A., Masson, P. (2016). Assessing Gravitropic Responses in Arabidopsis . In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_2
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DOI: https://doi.org/10.1007/978-1-4939-3356-3_2
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