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Immunohisto- and Cytochemistry Analysis of Connexins

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Gap Junction Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1437))

Abstract

Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing, and/or protein expression level. Basically, fluorescent IHC is a short, simple, and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody–antigen recognition.

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Acknowledgements

This work was financially supported by the University Hospital of the Vrije Universiteit Brussel-Belgium (Willy Gepts Fonds UZ-VUB), the Fund for Scientific Research-Flanders (FWO grants G009514N and G010214N), the European Research Council (ERC Starting Grant 335476), the University of São Paulo-Brazil and the São Paulo Research Foundation (FAPESP SPEC grant 2013/50420-6).

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Authors and Affiliations

  1. Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP, Brazil

    Bruno Cogliati, Isabel Veloso Alves Pereira & Tereza Cristina Da Silva

  2. Department of In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel, Jette, Belgium

    Michaël Maes, Joost Willebrords, Sara Crespo Yanguas & Mathieu Vinken

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  1. Bruno Cogliati
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  2. Michaël Maes
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  3. Isabel Veloso Alves Pereira
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  4. Joost Willebrords
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  5. Tereza Cristina Da Silva
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  6. Sara Crespo Yanguas
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  7. Mathieu Vinken
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Corresponding author

Correspondence to Bruno Cogliati .

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Editors and Affiliations

  1. Department of in Vitro Toxicology and D, Vrije Universiteit Brussel, Brussels, Belgium

    Mathieu Vinken

  2. BHF Glasgow Cardiovascular Research, University of Glasgow, Glasgow, United Kingdom

    Scott R. Johnstone

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Cogliati, B. et al. (2016). Immunohisto- and Cytochemistry Analysis of Connexins. In: Vinken, M., Johnstone, S. (eds) Gap Junction Protocols. Methods in Molecular Biology, vol 1437. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3664-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3664-9_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3662-5

  • Online ISBN: 978-1-4939-3664-9

  • eBook Packages: Springer Protocols

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