Abstract
Due to high resolution and reproducibility, chromatin immunoprecipitation (ChIP) has been used as a standard tool to investigate epigenetic marks including modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.
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References
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Nagaki, K. (2016). Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes. In: Murata, M. (eds) Chromosome and Genomic Engineering in Plants. Methods in Molecular Biology, vol 1469. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4931-1_16
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DOI: https://doi.org/10.1007/978-1-4939-4931-1_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-4929-8
Online ISBN: 978-1-4939-4931-1
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