Abstract
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. A homogenous cell-based MMP assay was optimized and performed in a 1536-well plate format to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.
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Sakamuru, S., Attene-Ramos, M.S., Xia, M. (2016). Mitochondrial Membrane Potential Assay. In: Zhu, H., Xia, M. (eds) High-Throughput Screening Assays in Toxicology. Methods in Molecular Biology, vol 1473. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6346-1_2
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DOI: https://doi.org/10.1007/978-1-4939-6346-1_2
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6346-1
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