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Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems

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Genotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1492))

Abstract

Microsatellites are polymorphic DNA loci comprising repeated sequence motifs of two to five base pairs which are dispersed throughout the genome. Genotyping of microsatellites is a widely accepted tool for diagnostic and research purposes such as forensic investigations and parentage testing, but also in clinics (e.g. monitoring of bone marrow transplantation), as well as for the agriculture and food industries. The co-amplification of several short tandem repeat (STR) systems in a multiplex reaction with simultaneous detection helps to obtain more information from a DNA sample where its availability may be limited. Here, we introduce and describe this commonly used genotyping technique, providing an overview on available resources on STRs, multiplex design, and analysis.

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Acknowledgements

We would like to cordially thank Peter Kovacs, head of the research group Genetics of Obesity and Diabetes, and our colleagues for their everlasting scientific and personal support. We thank Mohammed Hankir for proofreading of this manuscript.

Funding

Jacqueline Krüger is funded by a Collaborative Research Center (B03, CRC1052) granted by the German Research Foundation (DFG). Dorit Schleinitz is funded by the Boehringer Ingelheim Foundation.

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Authors and Affiliations

  1. Department of Medicine, Dermatology and Neurology, University of Leipzig, Leipzig, Germany

    Jacqueline Krüger & Dorit Schleinitz Ph.D.

  2. Leipzig University Medical Center, IFB Adiposity Diseases, University of Leipzig, Leipzig, Germany

    Jacqueline Krüger & Dorit Schleinitz Ph.D.

Authors
  1. Jacqueline Krüger
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  2. Dorit Schleinitz Ph.D.
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Correspondence to Dorit Schleinitz Ph.D. .

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Editors and Affiliations

  1. Leiden Genome Technology Center Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands

    Stefan J. White

  2. Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA

    Stuart Cantsilieris

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Krüger, J., Schleinitz, D. (2017). Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems. In: White, S., Cantsilieris, S. (eds) Genotyping. Methods in Molecular Biology, vol 1492. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6442-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6442-0_1

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