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Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution

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Antibiotics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1520))

Abstract

To elucidate the mechanisms of antibiotic resistance, integrating phenotypic and genotypic features in resistant strains is important. Here, we describe the expression profiling of antibiotic-resistant Escherichia coli strains obtained by laboratory evolution, and a method for extracting a small number of genes whose expression changes can contribute to the acquisition of resistance.

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Acknowledgment

This work was supported in part by a Grant-in-Aid for Scientific Research (S) [15H05746], a Grant-in-Aid for Scientific Research (B) [26290071,15H04733], and a Grant-in-Aid for Challenging Exploratory Research [26650138] from JSPS.

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Authors and Affiliations

  1. Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 565-0874, Japan

    Shingo Suzuki, Takaaki Horinouchi & Chikara Furusawa

Authors
  1. Shingo Suzuki
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  2. Takaaki Horinouchi
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  3. Chikara Furusawa
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Corresponding author

Correspondence to Chikara Furusawa .

Editor information

Editors and Affiliations

  1. Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany

    Peter Sass

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Suzuki, S., Horinouchi, T., Furusawa, C. (2017). Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution. In: Sass, P. (eds) Antibiotics. Methods in Molecular Biology, vol 1520. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6634-9_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6634-9_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6632-5

  • Online ISBN: 978-1-4939-6634-9

  • eBook Packages: Springer Protocols

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