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Proteomic Analysis of Dental Tissue Microsamples

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Oral Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1537))

Abstract

Improved understanding of dental enamel development will benefit not only dentistry but also biomedicine more generally. Rat and mouse models of enamel development are relatively well characterized and experimentally powerful. However, the diminutive size of murine teeth makes them difficult to study using standard proteomics approaches. Here, we describe gel-based proteomic methods that enable parallel quantification, identification, and functional characterization of proteins from developing rat and mouse teeth. These refined methods are applicable to other scarce samples including human enamel defects.

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Acknowledgments

We thank Nicola McHugh for skilfully assisting with development of the 2DGE procedures described here. This work was supported by the Melbourne Research Unit for Facial Disorders, the National Health and Medical Research Council of Australia, and the Health Research Council of New Zealand.

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia

    Jonathan E. Mangum, Jew C. Kon & Michael J. Hubbard

  2. Department of Pediatrics, Royal Children’s Hospital, University of Melbourne, Flemington Road, Melbourne, VIC, 3052, Australia

    Jew C. Kon & Michael J. Hubbard

Authors
  1. Jonathan E. Mangum
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  2. Jew C. Kon
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  3. Michael J. Hubbard
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Corresponding author

Correspondence to Michael J. Hubbard .

Editor information

Editors and Affiliations

  1. University of Otago Fac. Dentistry, Dunedin, New Zealand

    Gregory J. Seymour

  2. Department of Oral Sciences, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Mary P. Cullinan

  3. Sir John Walsh Research Institute, University of Otago Faculty of Dentistry, Dunedin, New Zealand

    Nicholas C.K. Heng

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Mangum, J.E., Kon, J.C., Hubbard, M.J. (2017). Proteomic Analysis of Dental Tissue Microsamples. In: Seymour, G., Cullinan, M., Heng, N. (eds) Oral Biology. Methods in Molecular Biology, vol 1537. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6685-1_28

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  • DOI: https://doi.org/10.1007/978-1-4939-6685-1_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6683-7

  • Online ISBN: 978-1-4939-6685-1

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