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Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering

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Adult Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1553))

Abstract

In this chapter, we introduce a completely intraoperative procedure for obtaining a patient-derived biomaterial in cell therapy and tissue engineering applications. An automated device for processing human peripheral-blood ensures a reproducible method for retrieving the patient’s cellular-rich as well as cellular-poor plasma. By substituting calcium for animal-derived thrombin, we engineer a completely autologous hydrogel that eliminates the risk of disease transmission and lowers FDA regulation hurdles. Through this chapter, we will discuss a bedside protocol developed to prepare a patient-derived hydrogel. This method can be effectively used to develop a completely intraoperative tissue engineering strategy (CITES) that can be easily translated into the clinic for surgical use.

*Authors contributed equally.

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Acknowledgments

Dr. Nukavarapu acknowledges funding from AO Foundation (S-13-122N), Musculoskeletal Transplant Foundation (MTF), and National Science Foundation (PFI AIR-131190 & EFRI-1332329). He also acknowledges support from Connecticut Institute for Clinical and Translational Science (CICATS) through a Mentorship award (M-1). Melissa thanks Young Innovative Investigator Program (YIIP), CICATS for fellowship. The authors are grateful to Deborah and Paiyz for their help with Magellan® System and confocal imaging.

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Author notes

    Authors and Affiliations

    1. Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA

      Shalmli U. Joshi, Rares O. Barbu & Syam P. Nukavarapu

    2. Institute for Regenerative Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA

      Melissa Carr-Reynolds & Syam P. Nukavarapu

    3. Arteriocyte, Cellular Therapies Medical Systems, Hopkinton, MA, 01748, USA

      Brian Barnes

    4. Materials Science & Engineering, University of Connecticut, Storrs, CT, 06269, USA

      Syam P. Nukavarapu

    5. Department of Orthopedic Surgery, University of Connecticut Health Center, Farmington, CT, 06030, USA

      Syam P. Nukavarapu

    Authors
    1. Shalmli U. Joshi
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    2. Rares O. Barbu
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    3. Melissa Carr-Reynolds
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    4. Brian Barnes
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    5. Syam P. Nukavarapu
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    Corresponding author

    Correspondence to Syam P. Nukavarapu .

    Editor information

    Editors and Affiliations

    1. Center for Regenerative Medicine, UniversitĂ  di Roma Tor Vergata, Rome, Italy

      Paolo Di Nardo

    2. Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

      Sanjiv Dhingra

    3. Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA

      Dinender K. Singla

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    Joshi, S.U., Barbu, R.O., Carr-Reynolds, M., Barnes, B., Nukavarapu, S.P. (2017). Patient-Derived and Intraoperatively Formed Biomaterial for Tissue Engineering. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_21

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    • DOI: https://doi.org/10.1007/978-1-4939-6756-8_21

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-6754-4

    • Online ISBN: 978-1-4939-6756-8

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