Abstract
Elimination of damaged or surplus mitochondria is crucial to maintain cellular integrity and an energy supply-demand balance. Mitophagy serves to selectively catabolize mitochondria in a manner dependent on autophagy, and contributes to mitochondrial quality and quantity control. This degradation system is highly conserved among eukaryotes including the budding yeast, Saccharomyces cerevisiae. Therefore, analyses of mitophagy using yeast have the potential to provide insights into the common mechanisms of mitophagy. Here, we introduce experimental approaches with fluorescence microscopy and western blotting to validate mitophagy in yeast. The former is useful to visualize transport of mitochondria to the vacuole, a lytic compartment, and formation of mitochondria-containing autophagosomes. The latter allows us to quantify mitochondria degradation.
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Acknowledgments
We thank Noriko Kondo-Okamoto for generating yeast strains and optimizing the original methods. This work was supported in part by Japan Society for the Promotion of Science (JSPS), and by the Ministry of Education, Culture, Sports, Science, and Technology. A.E. was supported by a JSPS Research Fellowship DC1 for Young Scientists.
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Eiyama, A., Okamoto, K. (2017). Assays for Mitophagy in Yeast. In: Mokranjac, D., Perocchi, F. (eds) Mitochondria. Methods in Molecular Biology, vol 1567. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6824-4_20
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DOI: https://doi.org/10.1007/978-1-4939-6824-4_20
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