Abstract
Reverse cholesterol transport (RCT) is one of the main processes that is thought to protect against cardiovascular disease. RCT constitutes the removal of cholesterol from peripheral sites, its transport through the plasma compartment for delivery to the liver for excretion. Here, we describe an in vivo RCT method that incorporates these steps, measuring movement of cholesterol from macrophages to the plasma, the liver, and finally to the feces in mice.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6875-6_22
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6875-6_22
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References
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Acknowledgments
This work was supported by the National Heart Foundation (Grant in aid G10S5192) and the National Health and Medical Research Council of Australia (Program grant 1037903).
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Kockx, M., Jessup, W., Kritharides, L. (2017). Measurement of Macrophage-Specific In Vivo Reverse Cholesterol Transport in Mice. In: Gelissen, I., Brown, A. (eds) Cholesterol Homeostasis. Methods in Molecular Biology, vol 1583. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6875-6_21
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DOI: https://doi.org/10.1007/978-1-4939-6875-6_21
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6873-2
Online ISBN: 978-1-4939-6875-6
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