Abstract
During assembly of the bacterial flagellum, structural subunits synthesized inside the cell must be exported across the cytoplasmic membrane before they can crystallize into the nascent flagellar structure. This export process is facilitated by a specialized Flagellar Type III Secretion System (fT3SS) located at the base of each flagellum. Here, we describe three methods—isothermal titration calorimetry, photo-crosslinking using unnatural amino acids, and a subunit capture assay—used to investigate the interactions of flagellar structural subunits with the membrane export machinery component FlhB.
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Acknowledgments
Research in the Fraser laboratory is supported by grant BB/M007197/1 from the Biotechnology and Biological Sciences Research Council. Paul Bergen is supported by a postgraduate scholarship from Gates Cambridge.
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Evans, L.D.B., Bergen, P.M., Bryant, O.J., Fraser, G.M. (2017). Interactions of Flagellar Structural Subunits with the Membrane Export Machinery. In: Minamino, T., Namba, K. (eds) The Bacterial Flagellum. Methods in Molecular Biology, vol 1593. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6927-2_2
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DOI: https://doi.org/10.1007/978-1-4939-6927-2_2
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