Abstract
Imaging mass spectrometry (IMS) is a novel quantitative technique used to investigative diverse biomolecules in tissue sections. Specifically, IMS uses analytical separation of mass spectrometry to determine the spatial distribution of certain lipids and/or proteins located directly on biological sections from a single tissue sample. Typically, IMS is combined with histological analysis to reveal additional distribution details of characterized biomolecules including cell type and/or subcellular localization. In this chapter, we describe the use of Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-Of-Flight/Time-Of-Flight (TOF/TOF) to analyze various cholesterol and phosphatidylcholine species in atherosclerotic plaque of murine heart aortic valves. In particular, we detail animals used, tissue collection, preparation, matrix application, spectra acquisition for generating a color-coded image based on IMS spectral characteristics.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chughtai K, Heeren RM (2010) Mass spectrometric imaging for biomedical tissue analysis. Chem Rev 110(5):3237–3277. doi:10.1021/cr100012c
Solon EG, Schweitzer A, Stoeckli M, Prideaux B (2010) Autoradiography, MALDI-MS, and SIMS-MS imaging in pharmaceutical discovery and development. AAPS J 12(1):11–26. doi:10.1208/s12248-009-9158-4
Berry KA, Li B, Reynolds SD, Barkley RM, Gijon MA, Hankin JA, Henson PM, Murphy RC (2011) MALDI imaging MS of phospholipids in the mouse lung. J Lipid Res 52(8):1551–1560. doi:10.1194/jlr.M015750
Norris JL, Caprioli RM (2013) Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research. Chem Rev 113(4):2309–2342. doi:10.1021/cr3004295
Le CH, Han J, Borchers CH (2013) Dithranol as a matrix for matrix assisted laser desorption/ionization imaging on a fourier transform ion cyclotron resonance mass spectrometer. J Vis Exp 81:e50733. doi:10.3791/50733
Meir KS, Leitersdorf E (2004) Atherosclerosis in the apolipoprotein-E-deficient mouse: a decade of progress. Arterioscler Thromb Vasc Biol 24(6):1006–1014. doi:10.1161/01.ATV.0000128849.12617.f4
Jawien J, Nastalek P, Korbut R (2004) Mouse models of experimental atherosclerosis. J Physiol Pharmacol 55(3):503–517
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Edwards, G., Mesa, A., Vazquez-Padron, R.I., Kowalski, JM., Bhattacharya, S.K. (2017). Sample Preparation and Analysis for Imaging Mass Spectrometry. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6996-8_5
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6995-1
Online ISBN: 978-1-4939-6996-8
eBook Packages: Springer Protocols