Abstract
Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols.
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Acknowledgments
The author gratefully acknowledges Dr Simona Francese for her support and guidance, and GlaxoSmithKline for the BBSRC Industrial Case Award funding.
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Patel, E. (2017). Fresh Frozen Versus Formalin-Fixed Paraffin Embedded for Mass Spectrometry Imaging. In: Cole, L. (eds) Imaging Mass Spectrometry . Methods in Molecular Biology, vol 1618. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7051-3_2
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DOI: https://doi.org/10.1007/978-1-4939-7051-3_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7050-6
Online ISBN: 978-1-4939-7051-3
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