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Plantform Bioreactor for Mass Micropropagation of Date Palm

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Date Palm Biotechnology Protocols Volume I

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1637))

Abstract

A novel protocol for the commercial production of date palm through micropropagation is presented. This protocol includes the use of a semisolid medium alternation or in combination with a temporary immersion system (TIS, Plantform bioreactor) in date palm micropropagation. The use of the Plantform bioreactor for date palm results in an improved multiplication rate, reduced micropropagation time, and improved weaning success. It also reduces the cost of saleable units and thus improves economic return for commercial micropropagation. The use of the Plantform bioreactor successfully addresses other hindrances that can occur during the scale-up of date palm micropropagation, including asynchrony of somatic embryos, limited maturation of somatic embryos, and highly variable germination frequencies of embryos.

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Author information

Authors and Affiliations

  1. Cell and Biotechnology Research Unit, College of Science, Basra University, Basra, Iraq

    Abdulminam H. A. Almusawi

  2. TC Propagation Ltd., Enniscorthy, Co. Wexford, Ireland

    Abdullah J. Sayegh

  3. Date Palm Research Centre, Basra University, Basra, Iraq

    Ansam M. S. Alshanaw

  4. Marine and Ecological Sciences, Florida Gulf Coast University, Ft. Myers, FL, USA

    John L. Griffis Jr

Authors
  1. Abdulminam H. A. Almusawi
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  2. Abdullah J. Sayegh
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  3. Ansam M. S. Alshanaw
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  4. John L. Griffis Jr
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Corresponding author

Correspondence to Abdullah J. Sayegh .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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Almusawi, A.H.A., Sayegh, A.J., Alshanaw, A.M.S., Griffis, J.L. (2017). Plantform Bioreactor for Mass Micropropagation of Date Palm. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7156-5_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7155-8

  • Online ISBN: 978-1-4939-7156-5

  • eBook Packages: Springer Protocols

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