Abstract
Liver perfusion is a common technique used to isolate parenchymal and non-parenchymal liver cells for in vitro experiments. This method allows hepatic cells to be separated based on their size and weight, by centrifugation using a density gradient. To date, other methods allow the isolation of only one viable hepatic cellular fraction from a single mouse; either parenchymal (hepatocytes) or non-parenchymal cells (i.e., Kupffer cells or hepatic stellate cells). Here, we describe a method to isolate both hepatocytes and Kupffer cells from a single mouse liver, thereby providing the unique advantage of studying different liver cell types that have been isolated from the same organism.
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Acknowledgments
We are grateful to Ahmed Elewa for critically reading the manuscript. MA and the Karolinska Institutet/AstraZeneca (KI/ZA) Integrated CardioMetabolic Center (ICMC) are supported by funding from AstraZeneca. MA is also supported by the Swedish Research council. MT is supported by the Novo Nordisk Foundation through the Danish Diabetes Academy and OUH Research grant 15-A845.
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Aparicio-Vergara, M., Tencerova, M., Morgantini, C., Barreby, E., Aouadi, M. (2017). Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver. In: Borel, F., Mueller, C. (eds) Alpha-1 Antitrypsin Deficiency . Methods in Molecular Biology, vol 1639. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7163-3_16
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DOI: https://doi.org/10.1007/978-1-4939-7163-3_16
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