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Biocatalysis of Steroids with Mycobacterium sp. in Aqueous and Organic Media

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Microbial Steroids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1645))

Abstract

Mycobacterium sp. can convert steroids such as β-sitosterol, campesterol, and cholesterol into commercially interesting products. In aqueous systems, the biocatalysis is limited by the low solubility of the steroids in water. This may be overcome by the introduction of an organic phase acting as a substrate and/or product reservoir.

In this chapter, we describe the biocatalysis of β-sitosterol to 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) both in aqueous and organic–aqueous systems. In the latter case, both traditional organic solvents and green solvents are proposed.

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Acknowledgments

C.C.C.R. de Carvalho acknowledges Fundação para a Ciência e a Tecnologia, I.P. (FCT), Portugal, for financial support under program “FCT Investigator 2013” (IF/01203/2013/CP1163/CT0002).

Author information

Authors and Affiliations

  1. IBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal

    Carla C. C. R. de Carvalho & Pedro Fernandes

  2. Faculty of Engineering, Universidade Lusófona de Humanidades e Tecnologias, Av. Campo Grande 376, 1749-024, Lisbon, Portugal

    Pedro Fernandes

Authors
  1. Carla C. C. R. de Carvalho
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  2. Pedro Fernandes
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Corresponding author

Correspondence to Carla C. C. R. de Carvalho .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

  2. Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de Boecillo, Boecillo, Valladolid, Spain

    Ignacio Herráiz

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de Carvalho, C.C.C.R., Fernandes, P. (2017). Biocatalysis of Steroids with Mycobacterium sp. in Aqueous and Organic Media. In: Barredo, JL., Herráiz, I. (eds) Microbial Steroids. Methods in Molecular Biology, vol 1645. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7183-1_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7183-1_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7182-4

  • Online ISBN: 978-1-4939-7183-1

  • eBook Packages: Springer Protocols

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