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Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins

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Recombinant Glycoprotein Production

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1674))

Abstract

Therapeutic proteins are widely used to treat severe conditions, such as oncologic and metabolic diseases, and also for vaccination. They are recognized for its high biopotency, but their hydrophilicity and high molecular weight make its transport through membranes difficult to achieve. They may also suffer in vivo structural instability caused by a proteolytic cleavage, turning them biological inactive. Polyester-based nanoparticles are useful tools to overcome such problems, and deliver therapeutic proteins in a controlled and localized manner, decreasing the number of administrations needed and enhancing the therapeutic outcome. The biodegradable and biocompatible nature of such carriers makes them feasible to be administered by invasive and noninvasive routes. Since nanoparticles are produced in suspension form, they are usually lyophilized to increase its long-term storage stability. Therefore, the aim of this work is to propose an intuitive protocol for production and lyophilization of polyester-based nanoparticles for therapeutic proteins delivery. The characterization techniques to evaluate the nanoparticle and lyophilisate features, as well as the structural stability of the loaded protein are also described. For these purposes, PLGA nanoparticles and human insulin are used as models.

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Acknowledgments

Flávia Sousa would like to thank the Fundação para a Ciência e a Tecnologia (FCT), Portugal for financial support (Grant SFRH/BD/112201/2015). This work was also financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Inovação in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274).

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Authors and Affiliations

  1. UCIBIO, REQUIMTE, Department of Chemical Sciences—Applied Chemistry Lab, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-113, Porto, Portugal

    Pedro Fonte

  2. i3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal

    Flávia Sousa & Bruno Sarmento

  3. INEB—Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, 4200-135, Porto, Portugal

    Flávia Sousa & Bruno Sarmento

  4. CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra 1317, 4585-116, Gandra, PRD, Portugal

    Bruno Sarmento

Authors
  1. Pedro Fonte
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  2. Flávia Sousa
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  3. Bruno Sarmento
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Corresponding author

Correspondence to Bruno Sarmento .

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Editors and Affiliations

  1. Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil

    Virgínia Picanço-Castro

  2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Kamilla Swiech

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Fonte, P., Sousa, F., Sarmento, B. (2018). Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins. In: Picanço-Castro, V., Swiech, K. (eds) Recombinant Glycoprotein Production. Methods in Molecular Biology, vol 1674. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7312-5_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7312-5_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7311-8

  • Online ISBN: 978-1-4939-7312-5

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