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Mass Spectrometric Identification of Endogenous Peptides

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Peptidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1719))

Abstract

Peptidomics is an emerging field focused in the analysis of endogenous peptides. Naturally occurring peptides are often endogenously produced protein fragments. Cleavage of precursor proteins by proteases generates peptides that may gain specialized functions not ascribed to their precursors, and which could reflect the state of a cell under certain physiological conditions or pathological processes.

Since peptides are found in complex matrices (e.g., serum, tear, urine, cerebrospinal fluid), they need to be isolated from the matrix and concentrated before they can be analyzed on mass spectrometry. This chapter describes methods for sample preparation prior to mass spectrometry analysis. In addition, different peptide fragmentation techniques are described which are complementary when analyzing naturally occurring peptides by liquid chromatography coupled online to tandem mass spectrometry.

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Acknowledgments

This work has been carried out with the financial support of the Basque Government, Bizkaia County, and ETORTEK and ELKARTEK programs.

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Authors and Affiliations

  1. Proteomics Platform, ProteoRed-ISCIII, CIBERehd, CIC bioGUNE, Science and Technology Park of Bizkaia, Derio, Spain

    Mikel Azkargorta, Iraide Escobes, Ibon Iloro & Felix Elortza

Authors
  1. Mikel Azkargorta
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  2. Iraide Escobes
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  3. Ibon Iloro
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  4. Felix Elortza
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Corresponding author

Correspondence to Felix Elortza .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Bayern, Germany

    Michael Schrader

  2. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, USA

    Lloyd Fricker

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Azkargorta, M., Escobes, I., Iloro, I., Elortza, F. (2018). Mass Spectrometric Identification of Endogenous Peptides. In: Schrader, M., Fricker, L. (eds) Peptidomics. Methods in Molecular Biology, vol 1719. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7537-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7537-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7536-5

  • Online ISBN: 978-1-4939-7537-2

  • eBook Packages: Springer Protocols

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