Abstract
During mesenchymal cell motility, various actin regulators are recruited to the leading edge with exquisite precision in time and space to generate protrusion and retraction cycles. We present here an automated method, named CorRecD (from Correlation Recruitment Dynamics), which quantifies cell edge dynamics, protein recruitment and analyze their cross-correlation. The Wave Regulatory Complex (WRC), a master driver of protrusions, is used as a case-of-study. This biologist-friendly method relies on free software tools and can be applied to any fluorescently tagged protein of interest.
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Acknowledgments
This work was supported by Fondation ARC pour la Recherche sur le Cancer (PJA 20151203371 to M.C.P.), Ligue National contre le Cancer (RS14/75-54 to M.C.P.) and Institut national de la Santé et de la Recherche médicale (Inserm, PC201530 to M.C.P.). The authors acknowledge the France Bio-Imaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04, “Investments for the future”).
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Paul-Gilloteaux, P., Waharte, F., Singh, M.K., Parrini, M.C. (2018). A Biologist-Friendly Method to Analyze Cross-Correlation Between Protrusion Dynamics and Membrane Recruitment of Actin Regulators. In: Gautreau, A. (eds) Cell Migration. Methods in Molecular Biology, vol 1749. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7701-7_20
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DOI: https://doi.org/10.1007/978-1-4939-7701-7_20
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