Abstract
In this chapter, we describe a protocol to induce at a high rate the differentiation of brown/brown like adipocyte progenitors (BAPs) derived from human induced pluripotent stem cells (hiPSCs). We also describe culture conditions to maintain hiPSCs and to derive hiPSC-BAPs.
This novel culture system provides an unlimited source of human brown adipocytes and a unique means for studying events regulating the generation and recruitment of human BAPs.
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Hafner, AL., Mohsen-Kanson, T., Dani, C. (2018). Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_4
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DOI: https://doi.org/10.1007/978-1-4939-7799-4_4
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7797-0
Online ISBN: 978-1-4939-7799-4
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