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Micropropagation in the Twenty-First Century

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Despite more than a century of research on effective biotechnological methods, micropropagation continues to be an important tool for the large-scale production of clonal plantlets of several important plant species that retain genetic fidelity and are pest-free. In some cases, micropropagation is the only technique that supports the maintenance and promotes the economic value of specific agricultural species. The micropropagation of plants solved many phytosanitary problems and allowed both the expansion and access to high-quality plants for growers from different countries and economic backgrounds, thereby effectively contributing to an agricultural expansion in this and the last century. The challenges for micropropagation in the twenty-first century include cost reduction, enhanced efficiency, developing new technologies, and combining micropropagation with other systems/propagation techniques such as microcuttings, hydroponics, and aeroponics. In this chapter, we discuss the actual uses of micropropagation in this century, its importance and limitations, and some possible techniques that can effectively increase its wider application by replacing certain conventional techniques and technologies.

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Acknowledgments

JCC thanks CNPQ for the research fellowship No. 304174/2015-7.

Contribution Statements

LTSG contributed writing item 2.4 Bioreactors Systems to Reduce the Cost of Micropropagated Plantlets. JATS contributed writing items 2.1 LEDs, CCFLs, and Laser Light for Tissue Culture and 3.2 The Use of Micropropagation for Secondary Metabolite Production and with final revision of all the text. JCC contributed writing all other items in the chapter and final revision of the text.

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Authors and Affiliations

  1. Laboratory of Plant Physiology and Tissue Culture, Department of Biotechnology, Plant and Animal Production, Centro de Ciências Agrárias, Universidade Federal de São Carlos, Araras, SP, Brazil

    Jean Carlos Cardoso & Lee Tseng Sheng Gerald

  2. Kagawa, Japan

    Jaime A. Teixeira da Silva

Authors
  1. Jean Carlos Cardoso
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  2. Lee Tseng Sheng Gerald
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  3. Jaime A. Teixeira da Silva
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Editor information

Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Cardoso, J.C., Sheng Gerald, L.T., Teixeira da Silva, J.A. (2018). Micropropagation in the Twenty-First Century. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8593-7

  • Online ISBN: 978-1-4939-8594-4

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